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custom-made microarray pcr plates specific for human inflammatory pathways yahs-205zd:339325  (Qiagen)


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    Qiagen custom-made microarray pcr plates specific for human inflammatory pathways yahs-205zd:339325
    Custom Made Microarray Pcr Plates Specific For Human Inflammatory Pathways Yahs 205zd:339325, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Human+PCR+Microarray/custom+made+microarray+pcr+plates+specific+for+human+inflammatory+pathways+yahs+205zd+339325/pmc12124685-234-19-22
    Average 90 stars, based on 1 article reviews
    custom-made microarray pcr plates specific for human inflammatory pathways yahs-205zd:339325 - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Quorum-sensing control of sepsis in the coagulase-negative staphylococcal species Staphylococcus haemolyticus.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline (PBS) no Mg or Ca Gibco CAT#10010-023 Dulbecco’s PBS Gibco CAT#14190-144 Dextran Sigma-Aldrich CAT#D8802 Ficoll-Paque PLUS Cytiva CAT#17144002 Triton X-100 Sigma-Aldrich CAT#T8787-100ML Tetracycline Sigma-Aldrich CAT#T3383 Ampicillin sodium salt Sigma-Aldrich CAT#A9518 Chloramphenicol Sigma-Aldrich CAT#C0378 Anhydrotetracycline Sigma-Aldrich CAT#37919 Yeast extract Sigma-Aldrich CAT#41625 Tryptone BD CAT#211705 Sodium Chloride Sigma-Aldrich CAT#3624-01 Non-fat powdered milk Saco Foods N/A Fluo3-AM Invitrogen CAT#F1242 Gentamicin Gibco CAT#15710-064 Saline ICU Medical CAT#798309 Acetonitrile Honeywell CAT#349984X4L N-Formylated PSMα peptide Peptide 2.0 N/A N-Formylated PSMβ1 peptide Peptide 2.0 N/A N-Formylated PSMβ2 peptide Peptide 2.0 N/A N-Formylated PSMβ3 peptide Peptide 2.0 N/A Trifluoroacetic acid Sigma-Aldrich CAT#A116-50 Critical commercial assays Human IL-8/CXCL8 DuoSet ELISA kit R&D systems CAT#DY208 Mouse CXCL-1 DuoSet ELISA kit R&D systems CAT#DY453 Mouse TNF⍺ DuoSet ELISA kit R&D systems CAT#DY410 Ovation® Pico WTA System V2 Nugen CAT#3302 Gateway BP clonase Invitrogen CAT#11789-013 Cytotoxicity Detection Kit (LDH) Roche CAT#11644793001 SuperScript TM III Platinum TM SYBR TM Green One-Step qRT-PCR Kit Invitrogen CAT#11736-059 DNA/RNA Shield Ready-to-use solution Zymo Research CAT#R1100-50 5ʹ RACE System For Rapid Amplification of cDNA Ends kit Invitrogen CAT#18374058 T4 RNA ligase 1 NEB CAT#M0204S Calf-intestinal alkaline phosphatase NEB CAT#M0525S DNAse Kit Amersham CAT#27051401 RNeasy Mini Kit Qiagen CAT#74106 Plasmid Midi Kit Qiagen CAT#12145 Deposited data Raw and analyzed microarray data This paper GEO: GSE285447 Whole genome sequencing data This paper BIOPROJECT: PRJNA1255573 Experimental models: Organisms/strains Mouse C57BL-6Ncrl Charles River Laboratories Strain code 027, RRID: IMSR_CRL:027 Oligonucleotides See Table S1 (Continued on next page) 14 Cell Reports 44, 116349, October 28, 2025

    Article Title: Therapeutic effect of miR-30b-5p-loaded lentivirus on experimental autoimmune uveitis via inhibiting Notch signaling activation
    Article Snippet: Lymphocyte separating solution (Solarbio, Beijing, China); beta-mercaptoethanol, glutamine (Ameresco, USA); fetal bovine serum (NEST Biotech., Wuxi, China); RPMI 1640 medium (HyClone, USA); fetal bovine serum (NEST, Wuxi, China); FITC-CD4, PE-CD8, PE-IL-17, APC-CD25, PE-Foxp3 (eBioscience, USA); Annexin V-FITC/PI apoptosis detection kit, Fixation/Permeabilization Kit with BD GolgistopTM (BD Biosciences, USA); interphotoreceptor retinoid-binding protein (IRBP) peptide (residues 1177–1191, sequence: ADGSSWEGVGVVPDV), phosphate buffered saline (PBS, pH 7.2) (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China); Mycobacterium tuberculin H37RA (TB) (Difco, USA); complete Freund's adjuvant (CFA, Sigma, USA); RNA Tissue/Cell Rapid Extraction Kit, BCA protein assay kit and SPARKScript II miRNA 1st strand cDNA synthesis kit (Shandong Sparkjade Biological Co. Ltd., Jinan, China); ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech Co., Ltd., Nanjing, China); RNeasy Microarray Tissue Mini Kit, DNA Reverse Transcription Kit, 2 × SYBR Green I Kit (QIAGEN, Germany); miR-30b-5p-carrying lentivirus and miR-30b-5p control vector carryng lentivirus (Genomeditech, Shanghai, China); Rabbit monoclonal Notch1 antibody (Abcam, Shanghai, China); rat GAPDH Rabbit pAb, Dll4 antibody Rabbit pAb (Bioss, Beijing, China); human β-actin Rabbit pAb, Dll4 Rabbit pAb, RBPJ Recombinant Rabbit mAb antibody (Bioss, Beijing, China); rat Notch1, Dll4 ELISA Kits (Wuhan ColorfulGene Biological Technology Co., Ltd., Wuhan, China); rat IL-10, IL-17 ELISA kits (Shanghai Jianglai Biological Co. Ltd., Shanghai, China); FITC rat IgG2b κ Isotype Control, PE rat IgG2b κ Isotype Control, FITC-CD4, PE-CD8, PE-IL17, APC-CD25, PE-Foxp3 (BD Biosciences, USA); RBPJ Inhibitor-1 (TargetMol, USA); Annexin V-FITC Apoptosis Detection Kit, Enhanced mitochondrial membrane potential assay kit (Beyotime, Shanghai, China).

    Article Title:
    Article Snippet: Comparison with predicate: Similarities and Differences Item Device Predicate FluChip-8G Influenza A+B Assay (K182513) CDC Human Influenza Virus Real-Time PCR Diagnostic Panel (K172091) Measurand Influenza RNA Same Viruses Detected Influenza A and B viruses Same Influenza A subtype Differentiation • Seasonal influenza A/H1N1pdm09 • Seasonal influenza A/H3N2 • “Non-seasonal” influenza A • Same • Same • Influenza A/H5 (Asian Lineage) Influenza B lineage Differentiation • Influenza B/Victoria • Influenza B/Yamagata Same Assay Type Qualitative Same CLIA Complexity High Same Sample Type Nasal swab and nasopharyngeal swab specimens Upper respiratory tract specimens, including nasal swabs and nasopharyngeal swabs; lower respiratory tract specimens Sample Preparation Method Nucleic Acid Extraction Same Detection Technology Fluorescence Same Technological Principles RT-PCR followed by endpoint detection by microarray via nucleic acid probes and fluorescence Real-time RT-PCR via nucleic acid probes and fluorescence Nucleic Acid Extraction QIAamp DSP Virus Spin Kit • QIAamp Viral RNA Mini Kit, Qiagen • QIAcube with QIAamp Viral RNA Mini Kit • MagNA Pure Compact—Total Nucleic Acid Kit, Roche • MagNA Pure Compact—RNA Isolation Kit, Roche • MagNA Pure LC—RNA Isolation Kit II, Roche • NucliSENS easyMAG, bioMerieux Controls • Internal Control in each specimen • External positive control processed with each batch • External negative control processed with each batch Same Amplification Reagents Quanta Biosciences qScript One-Step RTPCR Kit • Quanta Biosciences qScript One-Step qRT-PCR Kit, low ROX • Invitrogen SuperScript III Platinum One-Step Quantitative RT-PCR Kit Similarities and Differences Item Device Predicate FluChip-8G Influenza A+B Assay (K182513) CDC Human Influenza Virus Real-Time PCR Diagnostic Panel (K172091) Results Interpretation Automated test interpretation via neural network-based algorithm using fluorescence intensities Manual test interpretation via Ct value determined from fluorescence intensities Instrumentation FluChip-8G Imaging System Applied Biosystems 7500 Dx Real-Time PCR Instrument K. Standard/Guidance Document Referenced (if applicable): None L. Test Principle: The FluChip-8G Influenza A+B Assay (FC8G assay) includes a multiplex primer mix to amplify the hemagglutinin (HA), neuraminidase (NA), matrix (M), non-structural (NS), and nucleoprotein (NP) gene segments of all influenza A viruses, as well as the hemagglutinin (HA) and neuraminidase (NA) gene segments of all influenza B viruses, and a portion of the 18s gene present in eukaryotic cells as an endogenous internal control.

    Article Title: Foxp3 and BATF cooperatively direct cis-regulatory programs and gene expression for effector Treg cell differentiation.
    Article Snippet: Article Foxp3 and BATF cooperatively direct cis-regulatory programs and gene expression for effector Treg cell differentiation

    Transfection:

    Article Title: Human DEAH-box helicase 8 regulates HSF1-mediated stress response and cancer-associated pre-mRNA splicing in tumour cells
    Article Snippet: .. U2OS human osteosarcoma cells were reverse-transfected with 50 nM human Druggable Genome SMARTpool siRNA library (Dharmacon) or individual siRNAs (Dharmacon or Qiagen) using HiPerFect transfection reagent (Qiagen). .. We selected 72 h post-transfection as the primary assay time point, consistent with standard practice in cancer cell siRNA viability screens and with guidance that protein depletion and phenotypic effects typically lag mRNA knockdown (48–96 h), making ~72 h a robust screening window recommended by the library manufacturer (Dharmacon).

    Polymerase Chain Reaction:

    Article Title: miR-1-3p Downregulation as a Consistent Biomarker for Atrial Fibrillation Burden in Patients with Sick Sinus Syndrome: A Multi-Sample Analysis.
    Article Snippet: This step was performed using the ABI 7500 Fast Real-Time System (Applied Biosystems, Waltham, MA, USA), with PCR cycling parameters: 15 min at 95 ◦C, followed by 40 cycles of 15 s at 94 ◦C, 30 s at 55 ◦C, and 30 s at 70 ◦C. .. The human fibrosis miScript miRNA PCR array (Cat # MIHS-117Z, Qiagen, Hilden, Germany) was used for miRNA microarray detection, analyzing 84 miRNAs that exhibit altered expression during fibrosis development. ..

    Microarray:

    Article Title: miR-1-3p Downregulation as a Consistent Biomarker for Atrial Fibrillation Burden in Patients with Sick Sinus Syndrome: A Multi-Sample Analysis.
    Article Snippet: This step was performed using the ABI 7500 Fast Real-Time System (Applied Biosystems, Waltham, MA, USA), with PCR cycling parameters: 15 min at 95 ◦C, followed by 40 cycles of 15 s at 94 ◦C, 30 s at 55 ◦C, and 30 s at 70 ◦C. .. The human fibrosis miScript miRNA PCR array (Cat # MIHS-117Z, Qiagen, Hilden, Germany) was used for miRNA microarray detection, analyzing 84 miRNAs that exhibit altered expression during fibrosis development. ..

    Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review
    Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), miRNeasy Mini Kit (QIAGEN, Valencia, CA, USA) , NanoDrop (Thermo Scientific, Wilmington, DE, USA) Microarray (Human miRNA Microarray 8 × 60K V19.0, Agilent Technologies, Santa Clara, CA, USA) TaqMan MicroRNA Assay (Applied Biosystems, Foster City, CA, USA) using qRT-PCR (7500 Real-Time PCR System, Applied Biosystems, Foster City, CA, USA). .. 3 , F. Zahran 2015 [ ] , Saliva , Whole unstimulated saliva , - , Condition: subjects refrained from eating, drinking, using chewing gum for at least one and a half hour prior sampling Preservation: - Temporary storage: - Long-term storage: - , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA), qRT-PCR (Applied Biosystems, 7500 Real-Time PCR System, Foster City, CA, USA).

    Expressing:

    Article Title: miR-1-3p Downregulation as a Consistent Biomarker for Atrial Fibrillation Burden in Patients with Sick Sinus Syndrome: A Multi-Sample Analysis.
    Article Snippet: This step was performed using the ABI 7500 Fast Real-Time System (Applied Biosystems, Waltham, MA, USA), with PCR cycling parameters: 15 min at 95 ◦C, followed by 40 cycles of 15 s at 94 ◦C, 30 s at 55 ◦C, and 30 s at 70 ◦C. .. The human fibrosis miScript miRNA PCR array (Cat # MIHS-117Z, Qiagen, Hilden, Germany) was used for miRNA microarray detection, analyzing 84 miRNAs that exhibit altered expression during fibrosis development. ..

    Preserving:

    Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review
    Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), miRNeasy Mini Kit (QIAGEN, Valencia, CA, USA) , NanoDrop (Thermo Scientific, Wilmington, DE, USA) Microarray (Human miRNA Microarray 8 × 60K V19.0, Agilent Technologies, Santa Clara, CA, USA) TaqMan MicroRNA Assay (Applied Biosystems, Foster City, CA, USA) using qRT-PCR (7500 Real-Time PCR System, Applied Biosystems, Foster City, CA, USA). .. 3 , F. Zahran 2015 [ ] , Saliva , Whole unstimulated saliva , - , Condition: subjects refrained from eating, drinking, using chewing gum for at least one and a half hour prior sampling Preservation: - Temporary storage: - Long-term storage: - , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA), qRT-PCR (Applied Biosystems, 7500 Real-Time PCR System, Foster City, CA, USA).

    TaqMan microRNA Assay:

    Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review
    Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), miRNeasy Mini Kit (QIAGEN, Valencia, CA, USA) , NanoDrop (Thermo Scientific, Wilmington, DE, USA) Microarray (Human miRNA Microarray 8 × 60K V19.0, Agilent Technologies, Santa Clara, CA, USA) TaqMan MicroRNA Assay (Applied Biosystems, Foster City, CA, USA) using qRT-PCR (7500 Real-Time PCR System, Applied Biosystems, Foster City, CA, USA). .. 3 , F. Zahran 2015 [ ] , Saliva , Whole unstimulated saliva , - , Condition: subjects refrained from eating, drinking, using chewing gum for at least one and a half hour prior sampling Preservation: - Temporary storage: - Long-term storage: - , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA), qRT-PCR (Applied Biosystems, 7500 Real-Time PCR System, Foster City, CA, USA).

    Quantitative RT-PCR:

    Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review
    Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), miRNeasy Mini Kit (QIAGEN, Valencia, CA, USA) , NanoDrop (Thermo Scientific, Wilmington, DE, USA) Microarray (Human miRNA Microarray 8 × 60K V19.0, Agilent Technologies, Santa Clara, CA, USA) TaqMan MicroRNA Assay (Applied Biosystems, Foster City, CA, USA) using qRT-PCR (7500 Real-Time PCR System, Applied Biosystems, Foster City, CA, USA). .. 3 , F. Zahran 2015 [ ] , Saliva , Whole unstimulated saliva , - , Condition: subjects refrained from eating, drinking, using chewing gum for at least one and a half hour prior sampling Preservation: - Temporary storage: - Long-term storage: - , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA), qRT-PCR (Applied Biosystems, 7500 Real-Time PCR System, Foster City, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review
    Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), miRNeasy Mini Kit (QIAGEN, Valencia, CA, USA) , NanoDrop (Thermo Scientific, Wilmington, DE, USA) Microarray (Human miRNA Microarray 8 × 60K V19.0, Agilent Technologies, Santa Clara, CA, USA) TaqMan MicroRNA Assay (Applied Biosystems, Foster City, CA, USA) using qRT-PCR (7500 Real-Time PCR System, Applied Biosystems, Foster City, CA, USA). .. 3 , F. Zahran 2015 [ ] , Saliva , Whole unstimulated saliva , - , Condition: subjects refrained from eating, drinking, using chewing gum for at least one and a half hour prior sampling Preservation: - Temporary storage: - Long-term storage: - , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA), qRT-PCR (Applied Biosystems, 7500 Real-Time PCR System, Foster City, CA, USA).



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    Qiagen human cytokine/chemokine rt2 profilertm pcr microarray
    Effects of melatonin and radiation on breast cancer miRNA expression <t>microarray.</t> Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.
    Human Cytokine/Chemokine Rt2 Profilertm Pcr Microarray, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Journal: Biomedicines

    Article Title: Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells

    doi: 10.3390/biomedicines10051088

    Figure Lengend Snippet: Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Article Snippet: We next analyzed the influence of melatonin on the expression of multiple genes and miRNAs involved in breast cancer using a gene microarray (Human Breast Cancer RT 2 Profiler TM PCR Array) and a Human Breast Cancer miRNA microarray (MIHS-109ZA, Qiagen, Germantown, MD, USA).

    Techniques: Expressing, Microarray, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Control