custom-made microarray pcr plates specific for human inflammatory pathways yahs-205zd:339325 (Qiagen)
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Custom Made Microarray Pcr Plates Specific For Human Inflammatory Pathways Yahs 205zd:339325, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline (PBS) no Mg or Ca Gibco CAT#10010-023 Dulbecco’s PBS Gibco CAT#14190-144 Dextran Sigma-Aldrich CAT#D8802 Ficoll-Paque PLUS Cytiva CAT#17144002 Triton X-100 Sigma-Aldrich CAT#T8787-100ML Tetracycline Sigma-Aldrich CAT#T3383 Ampicillin sodium salt Sigma-Aldrich CAT#A9518 Chloramphenicol Sigma-Aldrich CAT#C0378 Anhydrotetracycline Sigma-Aldrich CAT#37919 Yeast extract Sigma-Aldrich CAT#41625 Tryptone BD CAT#211705 Sodium Chloride Sigma-Aldrich CAT#3624-01 Non-fat powdered milk Saco Foods N/A Fluo3-AM Invitrogen CAT#F1242 Gentamicin Gibco CAT#15710-064 Saline ICU Medical CAT#798309 Acetonitrile Honeywell CAT#349984X4L N-Formylated PSMα peptide Peptide 2.0 N/A N-Formylated PSMβ1 peptide Peptide 2.0 N/A N-Formylated PSMβ2 peptide Peptide 2.0 N/A N-Formylated PSMβ3 peptide Peptide 2.0 N/A Trifluoroacetic acid Sigma-Aldrich CAT#A116-50 Critical commercial assays Human IL-8/CXCL8 DuoSet ELISA kit R&D systems CAT#DY208 Mouse CXCL-1 DuoSet ELISA kit R&D systems CAT#DY453 Mouse TNF⍺ DuoSet ELISA kit R&D systems CAT#DY410 Ovation® Pico WTA System V2 Nugen CAT#3302 Gateway BP clonase Invitrogen CAT#11789-013 Cytotoxicity Detection Kit (LDH) Roche CAT#11644793001 SuperScript TM III Platinum TM SYBR TM Green One-Step qRT-PCR Kit Invitrogen CAT#11736-059 DNA/RNA Shield Ready-to-use solution Zymo Research CAT#R1100-50 5ʹ RACE System For Rapid Amplification of cDNA Ends kit Invitrogen CAT#18374058 T4 RNA ligase 1 NEB CAT#M0204S Calf-intestinal alkaline phosphatase NEB CAT#M0525S DNAse Kit Amersham CAT#27051401 Article Title: Therapeutic effect of miR-30b-5p-loaded lentivirus on experimental autoimmune uveitis via inhibiting Notch signaling activation Article Snippet: Lymphocyte separating solution (Solarbio, Beijing, China); beta-mercaptoethanol, glutamine (Ameresco, USA); fetal bovine serum (NEST Biotech., Wuxi, China); RPMI 1640 medium (HyClone, USA); fetal bovine serum (NEST, Wuxi, China); FITC-CD4, PE-CD8, PE-IL-17, APC-CD25, PE-Foxp3 (eBioscience, USA); Annexin V-FITC/PI apoptosis detection kit, Fixation/Permeabilization Kit with BD GolgistopTM (BD Biosciences, USA); interphotoreceptor retinoid-binding protein (IRBP) peptide (residues 1177–1191, sequence: ADGSSWEGVGVVPDV), phosphate buffered saline (PBS, pH 7.2) (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China); Mycobacterium tuberculin H37RA (TB) (Difco, USA); complete Freund's adjuvant (CFA, Sigma, USA); RNA Tissue/Cell Rapid Extraction Kit, BCA protein assay kit and SPARKScript II miRNA 1st strand cDNA synthesis kit (Shandong Sparkjade Biological Co. 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Article Snippet: Article Foxp3 and BATF cooperatively direct cis-regulatory programs and gene expression for effector Treg cell differentiation Transfection:Article Title: Human DEAH-box helicase 8 regulates HSF1-mediated stress response and cancer-associated pre-mRNA splicing in tumour cells Article Snippet: .. U2OS human osteosarcoma cells were reverse-transfected with 50 nM human Druggable Genome SMARTpool siRNA library (Dharmacon) or individual siRNAs (Dharmacon or Qiagen) using Polymerase Chain Reaction:Article Title: miR-1-3p Downregulation as a Consistent Biomarker for Atrial Fibrillation Burden in Patients with Sick Sinus Syndrome: A Multi-Sample Analysis. Article Snippet: This step was performed using the ABI 7500 Fast Real-Time System (Applied Biosystems, Waltham, MA, USA), with PCR cycling parameters: 15 min at 95 ◦C, followed by 40 cycles of 15 s at 94 ◦C, 30 s at 55 ◦C, and 30 s at 70 ◦C. .. The Microarray:Article Title: miR-1-3p Downregulation as a Consistent Biomarker for Atrial Fibrillation Burden in Patients with Sick Sinus Syndrome: A Multi-Sample Analysis. Article Snippet: This step was performed using the ABI 7500 Fast Real-Time System (Applied Biosystems, Waltham, MA, USA), with PCR cycling parameters: 15 min at 95 ◦C, followed by 40 cycles of 15 s at 94 ◦C, 30 s at 55 ◦C, and 30 s at 70 ◦C. .. The Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), Expressing:Article Title: miR-1-3p Downregulation as a Consistent Biomarker for Atrial Fibrillation Burden in Patients with Sick Sinus Syndrome: A Multi-Sample Analysis. Article Snippet: This step was performed using the ABI 7500 Fast Real-Time System (Applied Biosystems, Waltham, MA, USA), with PCR cycling parameters: 15 min at 95 ◦C, followed by 40 cycles of 15 s at 94 ◦C, 30 s at 55 ◦C, and 30 s at 70 ◦C. .. The Preserving:Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), TaqMan microRNA Assay:Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), Quantitative RT-PCR:Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), Real-time Polymerase Chain Reaction:Article Title: Salivary MicroRNAs as Innovative Biomarkers for Diagnosis and Prediction of the Oral Potentially Malignant Disorders Transition Towards Oral Cancer: A Systematic Review Article Snippet: 1 , F. Momen-Heravi 2014 [ ] , Saliva , Whole unstimulated saliva , 8 , Condition: refrain from eating, drinking and oral hygiene procedures on the day of saliva collection; saliva was collected from 6 to 11 am, after a water rinse Preservation: - Temporary storage: all saliva samples were processed immediately Long-term storage: −80 °C , miRNeasy kit (Qiagen, Valencia, CA, USA) , NanoDrop Spectrophotometer ND2000 (NanoDrop Technologies, Wilmington, DE, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), NanoString nCounter miRNA expression assay (NanoString Technologies Seattle, WA, USA), TaqMan MicroRNA Assay (Applied Biosystems) using qRT-PCR (MJ Research PTC-200 Thermal Cycler, MJ Research). .. 2 , Jin-Seok Byun 2015 [ ] , Saliva , Whole unstimulated saliva , 5–10 , Condition: saliva was collected from 10 to 12 am, after a mechanical tooth and tongue cleansing, and a rinse with distilled water Preservation: - Temporary storage: - Long-term storage: - , TRIzol reagent (Invitrogen, Carlsbad, CA, USA), |
